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91.
辜承林 《华中科技大学学报(自然科学版)》1994,(12)
基于变分有限元法,建立了各向异性非线性媒质中三维位场边值问题多媒质单元有限元分析的标量和矢量位分析模型,推导了单元分析的实用分析格式。讨论结合两种典型的八节点六面体多媒质等参单元进行,所得分析结果已成功应用于电力变压器铁芯磁场的三维分析与计算。 相似文献
92.
黄土高原东南部宋家店黄土剖面L9中地磁记录异常 总被引:1,自引:0,他引:1
对黄土高原东南部三门峡地区宋家店黄土剖面L8~S12段详细的磁性地层学和岩石磁学研究表明:Matuyama/Brunhes(M/B)界线位于L8黄土层的底部,Jaramillo极性亚时的顶、底界线分别位于L10的中部和L12的底部.黄土和古土壤地层的磁组构保持了原生沉积组构的特点,所记录的剩磁没有遭受到明显的后期扰动.在L9黄土层中记录了2次地磁场异常,认为由重磁化的可能性较小,而磁极性漂移事件的解释更为合理.推算它们的年龄分别与Kamikatsura(0.886Ma)和Santa Rosa(0.922Ma)地磁漂移事件接近,相对古强度研究结果显示,上述地磁漂移事件期间地球磁场强度减弱. 相似文献
93.
Chromatin-modifying enzymes as modulators of reprogramming 总被引:2,自引:0,他引:2
Onder TT Kara N Cherry A Sinha AU Zhu N Bernt KM Cahan P Marcarci BO Unternaehrer J Gupta PB Lander ES Armstrong SA Daley GQ 《Nature》2012,483(7391):598-602
94.
Interpretations of major climatic and biological events in Earth history are, in large part, derived from the stable carbon isotope records of carbonate rocks and sedimentary organic matter. Neoproterozoic carbonate records contain unusual and large negative isotopic anomalies within long periods (10-100 million years) characterized by δ(13)C in carbonate (δ(13)C(carb)) enriched to more than +5 per mil. Classically, δ(13)C(carb) is interpreted as a metric of the relative fraction of carbon buried as organic matter in marine sediments, which can be linked to oxygen accumulation through the stoichiometry of primary production. If a change in the isotopic composition of marine dissolved inorganic carbon is responsible for these excursions, it is expected that records of δ(13)C(carb) and δ(13)C in organic carbon (δ(13)C(org)) will covary, offset by the fractionation imparted by primary production. The documentation of several Neoproterozoic δ(13)C(carb) excursions that are decoupled from δ(13)C(org), however, indicates that other mechanisms may account for these excursions. Here we present δ(13)C data from Mongolia, northwest Canada and Namibia that capture multiple large-amplitude (over 10 per mil) negative carbon isotope anomalies, and use these data in a new quantitative mixing model to examine the behaviour of the Neoproterozoic carbon cycle. We find that carbonate and organic carbon isotope data from Mongolia and Canada are tightly coupled through multiple δ(13)C(carb) excursions, quantitatively ruling out previously suggested alternative explanations, such as diagenesis or the presence and terminal oxidation of a large marine dissolved organic carbon reservoir. Our data from Namibia, which do not record isotopic covariance, can be explained by simple mixing with a detrital flux of organic matter. We thus interpret δ(13)C(carb) anomalies as recording a primary perturbation to the surface carbon cycle. This interpretation requires the revisiting of models linking drastic isotope excursions to deep ocean oxygenation and the opening of environments capable of supporting animals. 相似文献
95.
96.
Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma 总被引:3,自引:0,他引:3
Schwartzentruber J Korshunov A Liu XY Jones DT Pfaff E Jacob K Sturm D Fontebasso AM Quang DA Tönjes M Hovestadt V Albrecht S Kool M Nantel A Konermann C Lindroth A Jäger N Rausch T Ryzhova M Korbel JO Hielscher T Hauser P Garami M Klekner A Bognar L Ebinger M Schuhmann MU Scheurlen W Pekrun A Frühwald MC Roggendorf W Kramm C Dürken M Atkinson J Lepage P Montpetit A Zakrzewska M Zakrzewski K Liberski PP Dong Z Siegel P Kulozik AE Zapatka M Guha A Malkin D Felsberg J Reifenberger G 《Nature》2012,482(7384):226-231
Glioblastoma multiforme (GBM) is a lethal brain tumour in adults and children. However, DNA copy number and gene expression signatures indicate differences between adult and paediatric cases. To explore the genetic events underlying this distinction, we sequenced the exomes of 48 paediatric GBM samples. Somatic mutations in the H3.3-ATRX-DAXX chromatin remodelling pathway were identified in 44% of tumours (21/48). Recurrent mutations in H3F3A, which encodes the replication-independent histone 3 variant H3.3, were observed in 31% of tumours, and led to amino acid substitutions at two critical positions within the histone tail (K27M, G34R/G34V) involved in key regulatory post-translational modifications. Mutations in ATRX (α-thalassaemia/mental retardation syndrome X-linked) and DAXX (death-domain associated protein), encoding two subunits of a chromatin remodelling complex required for H3.3 incorporation at pericentric heterochromatin and telomeres, were identified in 31% of samples overall, and in 100% of tumours harbouring a G34R or G34V H3.3 mutation. Somatic TP53 mutations were identified in 54% of all cases, and in 86% of samples with H3F3A and/or ATRX mutations. Screening of a large cohort of gliomas of various grades and histologies (n = 784) showed H3F3A mutations to be specific to GBM and highly prevalent in children and young adults. Furthermore, the presence of H3F3A/ATRX-DAXX/TP53 mutations was strongly associated with alternative lengthening of telomeres and specific gene expression profiles. This is, to our knowledge, the first report to highlight recurrent mutations in a regulatory histone in humans, and our data suggest that defects of the chromatin architecture underlie paediatric and young adult GBM pathogenesis. 相似文献
97.
Page MJ Symeonidis M Vieira JD Altieri B Amblard A Arumugam V Aussel H Babbedge T Blain A Bock J Boselli A Buat V Castro-Rodríguez N Cava A Chanial P Clements DL Conley A Conversi L Cooray A Dowell CD Dubois EN Dunlop JS Dwek E Dye S Eales S Elbaz D Farrah D Fox M Franceschini A Gear W Glenn J Griffin M Halpern M Hatziminaoglou E Ibar E Isaak K Ivison RJ Lagache G Levenson L Lu N Madden S Maffei B Mainetti G Marchetti L Nguyen HT O'Halloran B Oliver SJ Omont A Panuzzo P Papageorgiou A 《Nature》2012,485(7397):213-216
The old, red stars that constitute the bulges of galaxies, and the massive black holes at their centres, are the relics of a period in cosmic history when galaxies formed stars at remarkable rates and active galactic nuclei (AGN) shone brightly as a result of accretion onto black holes. It is widely suspected, but unproved, that the tight correlation between the mass of the black hole and the mass of the stellar bulge results from the AGN quenching the surrounding star formation as it approaches its peak luminosity. X-rays trace emission from AGN unambiguously, whereas powerful star-forming galaxies are usually dust-obscured and are brightest at infrared and submillimetre wavelengths. Here we report submillimetre and X-ray observations that show that rapid star formation was common in the host galaxies of AGN when the Universe was 2-6 billion years old, but that the most vigorous star formation is not observed around black holes above an X-ray luminosity of 10(44) ergs per second. This suppression of star formation in the host galaxy of a powerful AGN is a key prediction of models in which the AGN drives an outflow, expelling the interstellar medium of its host and transforming the galaxy's properties in a brief period of cosmic time. 相似文献
98.
Engineering the third wave of biocatalysis 总被引:1,自引:0,他引:1
Over the past ten years, scientific and technological advances have established biocatalysis as a practical and environmentally friendly alternative to traditional metallo- and organocatalysis in chemical synthesis, both in the laboratory and on an industrial scale. Key advances in DNA sequencing and gene synthesis are at the base of tremendous progress in tailoring biocatalysts by protein engineering and design, and the ability to reorganize enzymes into new biosynthetic pathways. To highlight these achievements, here we discuss applications of protein-engineered biocatalysts ranging from commodity chemicals to advanced pharmaceutical intermediates that use enzyme catalysis as a key step. 相似文献
99.
100.
Schmeisser MJ Ey E Wegener S Bockmann J Stempel AV Kuebler A Janssen AL Udvardi PT Shiban E Spilker C Balschun D Skryabin BV Dieck St Smalla KH Montag D Leblond CS Faure P Torquet N Le Sourd AM Toro R Grabrucker AM Shoichet SA Schmitz D Kreutz MR Bourgeron T Gundelfinger ED Boeckers TM 《Nature》2012,486(7402):256-260
Autism spectrum disorders comprise a range of neurodevelopmental disorders characterized by deficits in social interaction and communication, and by repetitive behaviour. Mutations in synaptic proteins such as neuroligins, neurexins, GKAPs/SAPAPs and ProSAPs/Shanks were identified in patients with autism spectrum disorder, but the causative mechanisms remain largely unknown. ProSAPs/Shanks build large homo- and heteromeric protein complexes at excitatory synapses and organize the complex protein machinery of the postsynaptic density in a laminar fashion. Here we demonstrate that genetic deletion of ProSAP1/Shank2 results in an early, brain-region-specific upregulation of ionotropic glutamate receptors at the synapse and increased levels of ProSAP2/Shank3. Moreover, ProSAP1/Shank2(-/-) mutants exhibit fewer dendritic spines and show reduced basal synaptic transmission, a reduced frequency of miniature excitatory postsynaptic currents and enhanced N-methyl-d-aspartate receptor-mediated excitatory currents at the physiological level. Mutants are extremely hyperactive and display profound autistic-like behavioural alterations including repetitive grooming as well as abnormalities in vocal and social behaviours. By comparing the data on ProSAP1/Shank2(-/-) mutants with ProSAP2/Shank3αβ(-/-) mice, we show that different abnormalities in synaptic glutamate receptor expression can cause alterations in social interactions and communication. Accordingly, we propose that appropriate therapies for autism spectrum disorders are to be carefully matched to the underlying synaptopathic phenotype. 相似文献